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Study on extraction, isolation and purification of β-ecdysteron from Cyanotis arachnoidea extract for reference standard establishment.
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Abstract
In Vietnam and China, Radix Achyranthis bidentatae has been widely used as a traditional herbal. With reference to the quality of Radix Achyranthis bidentatae, its content of β-ecdysteron quantified by HPLC/DAD is specified to be not less than 0.03 %. For the quality control of Radix Achyranthis bidentatae, the establishment of β-ecdysteron as an affordable-price reference standard is prerequisite in Vietnam. In this study, 1.1 g of β-ecdysteron was isolated and purified from 500 g of Cyanotis arachnoidea Extract. The structure of β-ecdysteron was confirmed by 1H, 13C-NMR, HR-MS, IR, UV-VIS…, as compared to the corresponding spectra of β-ecdysteron reference standard. The content of β-ecdysteron in our purified samples was determined
to be 92.4 % by HPLC-DAD analysis.
Article Details
Keywords
β-ecdysteron, Radix Achyranthis bidentatae, Cyanotis arachnoidea, extraction, isolation, purification
References
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