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Development of a method for simultaneous quantification of five phosphodiesterase type 5 (PDE-5) in postmortem blood samples by liquid chromatography tandem mass spectrometry
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Abstract
This study developed a method for the simultaneous qualitative and quantitative analysis of five selective phosphodiesterase type 5 (PDE-5) inhibitors, including sildenafil, tadalafil, vardenafil, avanafil, and thiosildenafil, in postmortem blood samples using liquid chromatography-mass spectrometry (LC-MS/MS). This method is developed based on current FDA guidelines. Results: Isotopes Warfarin-d5 was used as internal standard. The procedure for samples treatment was simple with the value of recovery being higher than 85 % and repeatability being lower than 15.0 %. The experimental results showed that the limit of detection (LOD) of the analytes ranged from 0.02 to 0.03 ng/mL. The linearity of the method was in the range of 1 ng/mL to 250 ng/mL with a good correlation coefficient (R2) from 0.9989 to 0.9993.
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Keywords
PDE-5, ildenafil, tadalafil, vardenafil, avanafil, thiosildenafil, post-mortem blood samples, LC-MS/MS
References
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