Nghiên cứu phân lập, tinh chế eurycomalacton từ dược liệu Mật nhân (Radix Eurycomae longifoliae) làm nguyên liệu thiết lập chuẩn

Abstract

Eurycomalactone (EUR), a quassinoide compound isolated from Radix Eurycoma longifolia, which were illustrated for anti-cancer, anti-inflammatory and anti-viral. This study has been developed an extraction and purification procedure of EUR for establishing its reference standard. In this procedure, the Radix Eurycoma longifolia powder was ultrasonically extracted with ethanol 50 % (v/v), and then ethanol was evaporated in vacuo to give water suspension. The suspension was successively partitioned with dichloromethane, eluted on silica column and crystallized in dichlromethan. Finally, the crystal was purified on C18 column to get EUR material. The EUR material, was identified by MS spectrometry, NMR, UVVIS and IR spectroscopy, contained 98.53 % of eurycomalactone (C₁₉H₂₄O₆), which was calculated from volatile impurities (0.15 %), residue on ignition (0.041 %) and the total of related substances (1.28 %). It was concluded that the established procedure was suitable for producing EUR material for reference standard establishment.