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Quantitative determination of aconitine in aconite root and black-processed aconite by hplc method
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Abstract
The procedure for quantifying aconitine in Fuzi and Black-processed aconite using the HPLC method has been successfully developed. The chromatographic conditions include an Agilent 1260 Infinity II HPLC system with a PDA detector, a Phenomenex C18 column (250 × 4.6 mm; 5 µm, USA), a flow rate of 1.0 mL/min, a sample injection volume of 20 μL, and chromatography performed at room temperature (25 ± 5 °C). The aconitine elution process is carried out according to a gradient program with a mobile phase system consisting of acetonitrile and a buffer solution containing 0.2 % glacial acetic acid, with the pH adjusted to 6.20 ± 0.05 using triethylamine. The quantification procedure has been validated to meet the requirements for system suitability, specificity, linearity, repeatability, intermediate precision, and accuracy. Quantitative results showed that Black-processed aconite has an aconitine content reduced by 4.5 times compared to Fuzi.
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Keywords
Aconite root, black-processed aconite, aconitine, HPLC
References
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