Xây dựng phương pháp định lượng đồng thời ba curcuminoid trong bã nghệ

Abstract

The bisdemethoxycurcumin (BDMC), demethoxycurcumin (DMC) and curcumin are main components of curcuminoid, already well known for their biological activities. In Vietnam, tumeric residues, side product in manufacturing tumeric starch and tumeric oil, still contain important amounts of curcuminoids but rarely exploited to fully making use of their usefulness. In this study, an HPLC method was developed for simultaneously assay of these three compounds in turmeric residue in order to evaluate remaining amount of curcuminoids for further employment. The analytes were completely seperated on a colunm C18 (250 x 4.6 mm, 5 µm) using a mixture of 0.1% solution of phosphoric acid and acetonitril (55 : 45, v/v) as mobile phase with the flow rate maintained at 1.5 ml/min, and UV detection at 425 nm. The method met the requirements of AOAC internationtal guideline for performance of analytical method in term of specificity, linearity, pricision, repeatability, and accuracy. The method was applied to determine curcuminoid content in five tumeric residue samples, finding the total amount of three analytes in these samples varying from 12.5 % to 14.5 % (w/w).