Article Sidebar
Views PDF: 0
Development and validation of a high performance liquid chromatography method for the determination of vitamin C in survival tablets
Main Article Content
Abstract
This study was conducted to develop and validate a high performance liquid chromatography (HPLC) method for the quantification of vitamin C in survival tablets. The optimized chromatographic conditions consisted of a C18 (250 x 4,6 mm, 5µm) column, a mobile phase of water containing 0,1% acetic acid and methanol (90 : 10, v/v), a flow rate of 0,5 mL/min, an injection volume of 10 µL, and a detection wavelength of 245 nm. Samples were processed by single ultrasonic extraction with a 0,1% TFA solution in water for 15 minutes. The method was validated according to ICH guidelines and showed good system suitability and high specificity. The method exhibited a linear range of 0,25 – 500 µg/mL with a correlation coefficient R2 = 0.9999. The limit of detection (LOD) and the limit of quantification (LOQ) were 0,10 µg/mL and 0,25 µg/mL, respectively. The precision of the method was satisfactory, with intra–day and inter–day RSD values below 2%, and recovery rates ranging from 95 to 105% (RSD < 2%). These results confirm that the method is suitable for the determination of vitamin C content in survival tablets.
Article Details
Keywords
Survival tablets, vitamin C, HPLC, method validation
References
2. Bộ Y tế (2018), Dược Điển Việt Nam V, Nhà xuất bản Y học.
3. Bộ Y tế (2007), Hóa Dược tập 1, Nhà xuất bản Y học.
4. Bộ Y tế (2007), Dược Lý Học tập 2, Nhà xuất bản Y học.
5. Correia, G., & Magina, S. (2023), Efficacy of topical vitamin C in melasma and photoaging: a systematic review, Journal of cosmetic dermatology, 22(7), 1938–1945.
6. Traber, M. G., & Stevens, J. F. (2011), Vitamins C and E: beneficial effects from a mechanistic perspective, Free radical biology & medicine, 51, 1000–1013.
7. N. Smirnoff (2018), Ascorbic acid metabolism and functions: A comparison of plants and mammals, Elsevier Inc, 122, 116-129.
8. Feszterová, M., Mišiaková, M., & Kowalska, M. (2023), Bioactive vitamin C content from natural selected fruit juices, Applied Sciences, 13(6), 3624.
9. L. T. Abe-Matsumoto, G. R. Sampaio, and D. H. M. Bastos (2020), Is titration as accurate as HPLC for determination of vitamin C insupplements?, Am J Analyt Chem, vol. 11, no. 07, pp. 269–279.
10. Sudhir, A., Sowmya, P. T., & Surekha, T. P. (2024), Determination of ascorbic acid in bulk and vitamin-C tablets using manganese in aqueous acidic perchlorate media, Pharm Pharmacol Int J, 12(2), 41-43.
11. Sowmya, P. T., Sudhir, A., Rai, K., & Deepakumari, H. N. (2020), Iodometric determination of ascorbic acid in bulk and Vitamin-C tablets using potassium iodate, Pharmacol. Int. J, 8, 159-161.
12. De Silva, D. S. P. C. N., Nonis, P. C. M., & Jayasundara, U. K. (2025), Method development and validation of vitamin C formulations using zeroth order uv-visible spectrophotometry, European Journal of Applied Sciences–Vol, 13(1).
13. Andaririt, D. R., Purnaningtyas, S. R. D., & Wijayanto, A. (2025), Validation of the uv-vis spectrophotometric method for the determination of ascorbic acid content in beverage preparations based on a standard vitamin C calibration curve, Open Access Health Scientific Journal, 6(2), 249-257.
14. Borras, E., Schrumpf, L., Stephens, N., Weimer, B. C., Davis, C. E., & Schelegle, E. S. (2021), Novel LC-MS-TOF method to detect and quantify ascorbic and uric acid simultaneously in different biological matrices, J Chromatogr B Analyt Technol Biomed Life Sci, vol. 1168, 122588.
15. Xing, Y., Zhao, B., Yin, L., Guo, M., Shi, H., Zhu, Z., Zhang, L., He, J., Ling, Y., Gao, M., Lu, H., Mao, E., & Zhang, L. (2021), Vitamin C supplementation is necessary for patients with coronavirus disease: an ultra-high-performance liquid chromatography-tandem mass spectrometry finding, J Pharm Biomed Anal, vol. 196, 113927.
16. Kakitani, A., Inoue, T., Matsumoto, K., Watanabe, J., Nagatomi, Y., & Mochizuki, N. (2014), Simultaneous determination of watersoluble
vitamins in beverages and dietary supplements by LC-MS/MS, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 31(12), 1939–1948.
17. Ibrahim, F., El-Enany, N., El-Shaheny, R. N., & Mikhail, I. E. (2015), Development and validation of a new HPLC method for the simultaneous determination of paracetamol, ascorbic acid, and pseudoephedrine HCl in their co-formulated tablets. application to in vitro dissolution testing, Analytical sciences: the international journal of the Japan Society for Analytical Chemistry, 31(9), 943–947.
18. Salah El-Din, S. H., Mahmoud, A. M., & Morsi, A. (2025), Eco-friendly RP-HPLC method for simultaneous determination of water-soluble and fat-soluble vitamins in nano-formula and pharmaceutical dosage forms, BMC chemistry, 19 (1), 1-9.
19. Alhat Babita, R., & Kokate Shekhar, V. (2019), Method development and validation for simultaneous estimation of l-glutathione and vitamin-C in effervescent tablet by RP-HPLC, Journal of Drug Delivery and Therapeutics, vol. 9, no. 4, pp. 348–353.
20. International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) (2025). ICH Q2(R2)/Q14 - Analytical Procedure Development and Validation, ICH, Geneva.
21. Ismillayli, N., Hermanto, D., Andayani, I. G. A. S., Honiar, R., Zuryati, U. K., Mariana, B., & Shofiyana, L. M. (2020), Determination of ascorbic acid content using the reverse phase–high performance liquid chromatography (RP-HPLC) method, Al-kimia, 8(2), 168-176.
22. Spínola, V., Mendes, B., Câmara, J. S., & Castilho, P. C. (2012), An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage, Analytical and bioanalytical chemistry, 403(4), 1049–1058.
23. Yuan, J. P., & Chen, F. (1998), Degradation of ascorbic acid in aqueous solution, Journal of Agricultural and Food Chemistry, 46(12), 5078-5082.
24. Wagner, B. A., & Buettner, G. R. (2023), Stability of aqueous solutions of ascorbate for basic research and for intravenous administration, Advances in redox research, 9, 100077.
25. Romeu-Nadal, M., Morera-Pons, S., Castellote, A. I., & López-Sabater, M. C. (2006), Rapid high-performance liquid chromatographic method for Vitamin C determination in human milk versus an enzymatic method, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 830(1), 41–46.