Article Sidebar
Views PDF: 7
Development of a method for simultaneous quantification of two alkaloids in human blood samples by liquid chromatography – tandem mass spectrometry
Main Article Content
Abstract
This study developed a method for the simultaneous qualitative and quantitative analysis of two alkaloids, including strychnine and brucine, in poisoning or post-mortem human blood samples using liquid chromatographymass spectrometry (LC-MS/MS). This method is developed based on current FDA guidelines. Results: Isotopes Warfarin-d5 was used as an internal standard. The procedure for samples treatment was simple, with the value of recovery being higher than 85% and repeatability being lower than 15.0%. The experimental results showed that the limit of detection (LOD) of strychnine and brucine were 0.25 ng/mL and 0.15 ng/mL. The linearity of the method was in the range of 1 ng/mL to 250 ng/mL with a correlation coefficient (R) from 0.9997 to 0.9998.
Article Details
Keywords
strychnine, brucine, alkaloid, human blood samples, LC-MS/MS
References
2. US FDA, “Bioanalytical method validation”, Guidance for industry. Rockville, MD: US Department of Health and Human Services Food and Drug Administration. 2018.
3. Chen Rui, Ning Ze, et al. (2019), “Simultaneous determination of 16 alkaloids in blood by ultrahigh-performance liquid chromatography-tandem mass spectrometry coupled with supported liquid extraction”, Journal of Chromatography B, 1128, pp. 121789.
4. Qiu Peihong, Chen Xiaohong, et al. (2008), “Simultaneous determination of five toxic alkaloids in body fluids by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry”, Journal of Chromatography B, 875(2), pp. 471-477.
5. Zhang Liang, Yu Wenying (2022), “Pharmacological effects, pharmacokinetics, and strategies to reduce brucine toxicity”, Revista Brasileira de Farmacognosia, 32(1), pp. 39-49.