Nghiên cứu một số độc tính của cao lỏng Desugan trên thực nghiệm

Abstract

This study aimed to evaluate acute oral toxicities on mice and subchronic toxicities on Wistar rats of Desugan liquid extracts. Both tests were conducted according to the guidelines of the Ministry of Health and OECD. The acute toxicity test’s results showed that, with the highest dose tested, 172.8 g of dry medicinal material/kg, no mice died after 7 days of monitoring. All mice remained healthy, ate, drank, moved, urinated and defecated normally and had ordinary nervous and respiratory functions. The weight of mice on day 7 increased remarkably compared to that on day 0 (p < 0.001). The results of the macroscopic observation of mice’s hearts, livers, kidneys, lungs, bladders and intestines did not show abnormal. In the second experiment, rats that took Desugan at a dose of 3.402 or 10.206 g/kg/day for 30 consecutive days had insignificant changes in biochemical and hematological indexes on days 15 and 30 compared to that on day 0 (p > 0.05). In addition,
macroscopic observations of rats’ liver and kidney tissues in all groups did not show abnormal. Nevertheless, microscopic observations revealed that 1/3 of rats (33.33%) in both groups receiving Desugan at a dose of 3.402 or 10.206 g/kg/day had mild fibrosis and granular degeneration of liver cells degeneration with no remarkable differences compared with that of the control group while the remaining (2/3, 66.67%) had ordinary liver tissues. Apart from that, only 1/3 of rats in the group receiving Desugan at a dose of 10.206 g/kg/day had its renal tubular and peritubular vascular congestion while the remaining rats in this group (2/3, 66,67%) and all rats (3/3, 100%) in the group receiving Desugan at a dose of 3.402 g/kg/day had normal renal tissues. Desugan did not affect rats’ physical conditions, hematopoietic, and biochemical indexes. Further studies are needed to conclude Desugan’s effects on the histology of rats’ livers and kidneys.