Xác định tồn dư kháng sinh erythromycin trong vắc xin sởi đông khô bằng phương pháp LC-MS/MS.

Abstract

A simple and sensitive method was developed for determination of residual erythromycin in lyophilized measles vaccine using liquid chromatography tandem mass spectrometry (LC-MS/MS). Erythromycin was extracted from vaccine with methanol. Liquid chromatography was performed on an Acquity BEH-C18; (100 x 2,1 mm; 1,7 μm) column with gradient program of 0.1 % aqueous solution of formic acid and acetonitrile containing 0.1 % formic acid as mobile phase at flow rate of 0.2 mL per minute, temperature: 40⁰C. The Waters Xevo TQD UPLC-MS/MS was operated under the multiple-reaction monitoring (MRM) mode using the ESI+ mode with transitions at (m/z) 734.5 → 157.9 and 734.5 → 576.4. The method was validated for the specificity, linear range, precision, accuracy, LOD and LOQ. The validation results showed that the method was suitable for determination of residual erythromycin in lyophilized measles vaccine.