Nghiên cứu xây dựng quy trình xác định chất cấm theophylin có nguy cơ trộn trái phép trong một số nền mỹ phẩm bằng phương pháp HPLC/DAD

Abstract

A reverse-phase liquid chromatography method was developed to detect and assay theophylline in the cream and the lotion. Chromatographic conditions are as follows: The liquid chromatograph is equipped with a 270 nm detector, a 250 x 4.6 mm column that contains 5 µm packing octadecyl silane, a mixture of methanol and water (25:75) as mobile phase, the flow rate of about 1.0 ml/min, injection volume is 20 µl, temperature: ambient.
The method was validated about the specificity, linear range, precision, accuracy, range, detection limit, and quantitation limit and validation results proved that this method was suitable for the determination of theophylline in the cream and lotion.