Xây dựng quy trình định lượng cefuroxim axetil trong viên nén zanimex có thành phần chưa biết bằng phương pháp quang phổ Raman

Abstract

A fast, simple Raman spectroscopic method was developed for quantitation of cefuroxim axetil in Zanimex tablets with unknown manufacturing formula. The method employed assay model based on spectral data of reference mixture having mass ratio of cefuroxim axetil over total amount of cefuroxim axetil and internal standard from 30% to 70% (w/w) recorded in Raman shift zones from 845 cm-1 to 880 cm-1, from 1460 cm-1 to 1505 cm-1, and from 1560 cm-1 to 1615 cm-1 with PLS algorithm. The method was validated according to current requirements on performance of analytical methods, and was proven to have suitable specificity, linearity, accuracy, and precision for intended application.