Xây dựng và thẩm định phương pháp LC-MS/MS định lượng paroxetin trong huyết tương người

Abstract

A fast, simple, high throughput and specific method using ultra performance liquid chromatography tandem mass spectrometry was developed for quantitation of paroxetine in human plasma. The Waters Xevo TQD UPLC-MS/MS was operated under the multi reaction-monitoring mode (MRM 330.1>>192.1 for paroxetine và MRM 334.1>>196.1 for paroxetine-d4) using positive electrospray ionization technique. Paroxetine and internal standard – paroxetine-d4 were extracted from human plasma by liquid-liquid extraction. The samples were chromatographed on a C18 column (dimention 50 x 3 mm, particle 2.5 µm) at a flow rate of 0.2 mL/min using 20 mM ammonium formate solution – acetonitrile at ratio of 15 : 85. The calibration curves were found to be linear in the range 0.05 to 20 ng/mL, with mean correlation coefficient of > 0.999. The accuracy was within 85% – 115.0% and the precision was less than 15%. Total run time was 2.5 min. This method can be used for BA-BE studies of paroxetine preparations.