Phát triển phương pháp HPLC định lượng dihydromyricetin trong dược liệu Chè dây và sản phẩm chứa dược liệu này

Abstract

This study aimed to develop a novel, simple, and specificity method for the quantification of
dihydromyricetin (DMY) in raw herbal materials and preparations containing Ampelopsis cantoniensis (Chè dây). The results showed that the samples were ultrasonically extracted twice with methanol, and the extracts were diluted tenfold with 0.01 M phosphoric acid. A standard solution of DMY at a concentration of 100 µg/mL was prepared simultaneously. Chromatographic separation was carried out on a C18 column (150 × 4.6 mm, 5 µm). The gradient program consisted of acetonitrile (ACN) and 0.01 M phosphoric acid as the mobile phases: 0 – 5 min, 5% ACN; 5 – 15 min, 5 – 22% ACN; 15 – 25 min, 90% ACN; 25 – 30 min, 5% ACN, at a flow rate of 1.0 mL/min. The injection volume was 10 µL, and detection was performed at 292 nm. Validation results met the requirements stipulated in the ICH and AOAC guidelines. The study successfully developed a new and straightforward method for the quantification of DMY in Ampelopsis cantoniensis materials and their preparations, contributing to improved quality control of these herbal products.